Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Nexturastat A 是一种有效且特异性的 HDAC6 抑制剂,IC50: 5 nM。它的选择性是其他 HDAC 的 190 倍以上。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 635 | 现货 | ||
5 mg | ¥ 1,890 | 现货 | ||
10 mg | ¥ 3,230 | 现货 | ||
25 mg | ¥ 5,330 | 现货 | ||
50 mg | ¥ 7,480 | 现货 | ||
100 mg | ¥ 9,870 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 1,430 | 现货 |
产品描述 | Nexturastat A is an effective and specific HDAC6 inhibitor (IC50: 5 nM). Its selectivity is >190-fold than other HDACs. |
靶点活性 | HDAC6:5 nM |
体内活性 | Nexturastat A对B16鼠黑色素瘤细胞的生长有显著抑制作用(GI50:14.3 μM)。在B16鼠黑色素瘤细胞中,Nexturastat A剂量依赖性增加乙酰化的α-微管蛋白水平,但不影响组蛋白H3乙酰化伴随的脓肿。 |
激酶实验 | HDAC inhibition assays: HDAC inhibition assays are performed by Reaction Biology Corp. using isolated human, recombinant full2length HDAC1 and -6 from a baculovirus expression system in Sf9 cells. An acetylated fluorogenic peptide,RHKKAc, derived from residues 379-382 of p53 is used as substrate. The reaction buffer is made up of 50 mM Tris-HCl pH 8.0, 127 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1 mg/mL BSA, and a final concentration of 1% DMSO. Compounds are delivered in DMSO and delivered to enzyme mixture with preincubation of 5-10 min followed by substrate addition and incubation for 2 h at 30°C. Trichostatin A and developer are added to quench the reaction and generate fluorescence, respectively. Dose-response curves are generated starting at 30 μM compound with three-fold serial dilutions to generate a 10-dose plot. IC50 values are then generated from the resulting plots, and the values expressed are the average of duplicate trials ± standard deviation. |
细胞实验 | B16 murine melanoma cells are plated at 5000/well in 96 well flat bottom plates. The following day, media is changed to that containing various concentrations of HDACi or matched DMSO vehicle concentrations diluted in complete medium done in triplicate. Cells are incubated for 48 hours at 37°C and 5% CO2. Density of viable, metabolically active cells is quantified using a standard MTS assay as per manufacturer’s instructions. Briefly, 20μL of reagent are added per well and incubated at 37°C for 3 hours. Absorbances at 490 nM are measured spectrophotometrically with background subtraction at 690 nM. All values are then normalized and expressed as a percentage of medium control (100%). (Only for Reference) |
分子量 | 341.4 |
分子式 | C19H23N3O3 |
CAS No. | 1403783-31-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 63 mg/mL (184.5 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: 2 mg/mL (5.85 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / Ethanol | 1 mM | 2.9291 mL | 14.6456 mL | 29.2912 mL | 73.2279 mL |
5 mM | 0.5858 mL | 2.9291 mL | 5.8582 mL | 14.6456 mL | |
DMSO | 10 mM | 0.2929 mL | 1.4646 mL | 2.9291 mL | 7.3228 mL |
20 mM | 0.1465 mL | 0.7323 mL | 1.4646 mL | 3.6614 mL | |
50 mM | 0.0586 mL | 0.2929 mL | 0.5858 mL | 1.4646 mL | |
100 mM | 0.0293 mL | 0.1465 mL | 0.2929 mL | 0.7323 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Nexturastat A 1403783-31-2 Chromatin/Epigenetic DNA Damage/DNA Repair HDAC inhibit Histone deacetylases Inhibitor inhibitor